Skip to main content
. 2014 Mar 20;25(9):2040–2052. doi: 10.1681/ASN.2013050466

Figure 2.

Figure 2.

The HC diet stimulated the ammoniagenic pathway. Kidneys collected from all three groups of mice after 4 or 9 days of the HC diet were analyzed by quantitative RT-PCR and immunoblotting. After 4 days of the HC diet, Rhcg+/+ highly increased mRNA (A) and protein (B) expression of SNAT3. Rhcg+/− and Rhcg −/− exhibited higher SNAT3 mRNA (A) and protein (E) levels than did Rhcg+/+. At day 9, only Rhcg−/− kept an enhanced level of SNAT3 mRNA (A) and protein (F). 4 or 9 days HC diet augmented Rhcg+/+ PDG mRNA (C) or protein (D) abundance. Rhcg−/− displayed higher mRNA (C) after 9 days HC and higher protein (G and H) expressions after both 4 and 9 days HC. Values are mean±SEM (n=4–8 mice) #P≤0.05 significantly different from same genotype under control conditions (day 0), *P≤0.05, **P≤0.01, ***P≤0.001 significantly different from Rhcg+/+ mice under the same treatment conditions and for the same time point.