Skip to main content
. 2014 Mar 20;25(9):2040–2052. doi: 10.1681/ASN.2013050466

Figure 4.

Figure 4.

The HC diet stimulates diuresis and regulates NKCC2 and AQP2 expression. Protein expression levels were examined using immunoblotting, and urine production was measured at different time points of the HC diet in Rhcg+/+ and Rhcg −/− mice. NKCC2 protein levels were decreased after 4 and 9 days of the HC diet in Rhcg+/+ (A) and only after 9 days in Rhcg−/− (C), while AQP2 expression was reduced in Rhcg+/+ (B) and Rhcg −/− (D) mice at day 9 of the HC diet. Rhcg−/− showed higher urinary excretion than Rhcg+/+ (E). Mature pSer256-AQP2 showed decreased abundance at day 9 in Rhcg−/−, and the ratio of pSer256-AQP2 over total AQP2 was reduced in Rhcg−/− (F). Values are mean±SEM (n=4–8 mice). #P≤0.05 significantly different from same genotype under control conditions (day 0); *P≤0.05, **P≤0.01; ***P≤0.001 significantly different from Rhcg+/+ mice under same treatment conditions and for the same time point.