Figure 5.

Rhcg^−/− mice develop hypercalcemia and hypercalciuria, and show signs of increased bone resorption during HC diet. (A) After 2 and 4 days of the HC diet, ionized Ca²⁺ concentration was higher in Rhcg^−/− blood. (B) At day 4, urinary Ca²⁺ excretion was also increased in Rhcg ^−/−. (C) The release of the bone degradation marker Dpd was augmented in Rhcg^−/− compared with Rhcg^+/+ following 4 and 9 days the HC diet, whereas the plasma concentration of the bone formation marker osteocalcin (D) was not affected by diet and genotype. Values are mean±SEM (n=5–8 mice). ^#P≤0.05 significantly different from same genotype under control conditions (day 0); *P≤0.05, **P≤0.01 significantly different from Rhcg^+/+ mice under same treatment conditions and for the same time point.