Table 1.
Body weight, food intake, and urinary values of Rhcg+/+, Rhcg+/−, and Rhcg−/− mice during 9 days of HC protein loading
| Variable | Basal Status | 2 Days of HC Diet | 4 Days of HC Diet | 9 Days of HC Diet | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Rhcg+/+ (n=8) | Rhcg+/− (n=8) | Rhcg−/− (n=8) | Rhcg+/+ (n=8) | Rhcg+/− (n=8) | Rhcg−/− (n=8) | Rhcg+/+ (n=8) | Rhcg+/− (n=8) | Rhcg−/− (n=8) | Rhcg+/+ (n=8) | Rhcg+/− (n=8) | Rhcg−/− (n=8) | |
| Body weight (g) | 28.2±0.6 | 29.7±0.4 | 27.0±0.8 | 28.0±0.5 | 29.5±0.7 | 26.0±0.9 | 27.6±0.6 | 29.0±0.7 | 25.7±0.9 | 27.8±0.4 | 27.3±0.9 | 25.9±0.9 |
| Food intake (g/24 h/body wt) | 0.14±0.02 | 0.14±0.03 | 0.15±0.02 | 0.10±0.02a | 0.08±0.04a | 0.10±0.04a | 0.10±0.02a | 0.10±0.03a | 0.10±0.02a | 0.12±0.02a | 0.10±0.05a | 0.10±0.02a |
| Water intake (g/24 h/body wt) | 0.16±0.01 | 0.14±0.01 | 0.15±0.01 | 0.24±0.03a | 0.21±0.02a | 0.21±0.02a | 0.22±0.03a | 0.23±0.02a | 0.26±0.03a | 0.25±0.03a | 0.24±0.03a | 0.26±0.03a |
| Urine values | ||||||||||||
| Volume (ml/24 h) | 1.89±0.32 | 1.91±0.18 | 1.74±0.19 | 4.51±0.44a | 4.34±0.29a | 5.20±0.37a | 4.24±0.50a | 4.38±0.38a | 5.523±0.55a | 4.52±0.17a | 4.36±0.24a | 5.65±0.30a,b |
| Urinary pH | 5.94±0.08 | 5.97±0.04 | 6.03±0.06 | 6.0±0.04 | 5.94±0.03 | 5.87±0.04 | 5.97±0.04 | 6.03±0.05 | 5.89±0.04 | 5.91±0.04 | 5.85±0.04 | 5.86±0.07 |
| NH4+/urine volume (mmol/24 h) | 0.028±0.01 | 0.028±0.01 | 0.027±0.01 | 0.26±0.08a | 0.24.0±0.07a | 0.16±0.04a,c | 0.29±0.08a | 0.24±0.07a | 0.17±0.05a,d | 0.28±0.08a | 0.28±0.05a | 0.29±0.08a |
| TA/urine volume (mmol/24h) | 0.08±0.06 | 0.09±0.03 | 0.07±0.03 | 0.22±0.09a | 0.25±0.05a | 0.22±0.06a | 0.20±0.08a | 0.23±0.06a | 0.23±0.08a | 0.24±0.07a | 0.28±0.06a | 0.27±0.05a |
| Ca2+/urine volume (μmol/24 h) | 1.5±0.1 | 1.6±0.1 | 1.2±0.1 | 3.3±0.1a | 3.2±0.7a | 6.5±0.2a,b | 5.4±0.3a | 5.5±0.2a | 8.2±0.4a,c | 4.1±0.4 | 6.8±0.2a | 5.1±0.4a |
| Dpd/urine volume (nmol/24 h) | 0.076±0.04 (n=6) | ND | 0.073±0.02 (n=6) | ND | ND | ND | 0.100±0.07 (n=6) | ND | 0.211±0.01a,c (n=6) | 0.093±0.02 (n=6) | ND | 0.149±0.05a,c (n=6) |
Body weight, food intake, and urinary values of Rhcg+/+, Rhcg+/−, and Rhcg−/− mice during 9 days HC protein loading (n=8 mice for each group). ND, not determined.
a
P≤0.05 significantly different from same genotype under control conditions.
b
P≤0.01 significantly different from Rhcg+/+ mice under same treatment conditions.
c
P≤0.05 significantly different from Rhcg+/+ mice under same treatment conditions.
d
P≤0.001 significantly different from Rhcg+/+ mice under same treatment conditions.