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. 2014 Mar 27;25(9):2067–2078. doi: 10.1681/ASN.2013070811

Figure 3.

Figure 3.

Diabetic TRB3 knockout mice have higher renal inflammatory gene expression and evidence of ER stress than WT controls. Real-time PCR on kidney cortices comparing diabetic with nondiabetic controls revealed the following: (A) increased TRB3 expression in the diabetic WT mice compared with nondiabetic WT mice and higher (B) MCP-1, (C) IL-6, (D) Lcn2/Ngal, and (E) fibronectin expression in diabetic TRB3−/− mice than in WT diabetic mice. (F) There was no difference in TGF-β mRNA expression in the diabetic WT and TRB3−/− mice. (G–I) There was higher renal cortical expression of the ER stress markers (GRP78/BiP and ATF4) and a trend for higher CHOP in the diabetic TRB3−/− mice. In both genotypes, there was higher excretion of (J) the early-response cytokine IL-6 in 24-hour urine collected 4 weeks after STZ administration and (K) MCP-1 and (L) Lcn2/Ngal in urine collected at 8 weeks after diabetes induction. *P<0.05 versus WT control mice, **P<0.05 versus TRB3−/− control mice; n=5 WT control, n=5 TRB3−/− control, n=7 WT STZ, n=12 TRB3−/− STZ. Bars represent SEM.