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. Author manuscript; available in PMC: 2014 Aug 28.
Published in final edited form as: Eur J Pharmacol. 2011 Jun 25;668(0):194–200. doi: 10.1016/j.ejphar.2011.06.019

Fig. 4. Dexamethasone causes elevated expression of Bcl-xL in the myocardium.

Fig. 4

C57BL6 mice at 8 weeks old were used for left anterior descending coronary artery occlusion surgery or dexamethasone treatment. For preconditioning, the coronary artery was occluded for 5 mins then released for 5 mins for two cycles. Ventricular tissues were collected immediately at the end of the ischema-reperfusion cycles for Western blot analyses (80 µg protein/lane) or RT-PCR (A). Dexamethasone (i.p. 20 mg/kg) was administered 20 h prior to ventricular tissue collection (B). Each lane represents Bcl-xL protein or mRNA level from one animal. The quantitative Bcl-xL protein data (C) or mRNA (D) show means + standard deviations of band densities from three animals. Vinculin (A) or GAPDH (B) was used as a loading control. * indicates p<0.05 and ** indicates p<0.01.