Figure 2.

Screening for microRNAs (miRNAs) with modulatory activity on the induction of Gli-luciferase reporter by Shh treatment. (A) Experimental procedure. (B) Luciferase reporter data analysis. (C) miRNAs with positive or negative regulatory activities on the induction of Gli-reporter by Shh in Shh-LIGHT2 cells. Numbers on the Y axis are Ln-transformed relative fold changes. (D) Expression of miRNAs that are influenced by Shh signaling activity. (E) The relative levels of miRNAs in control lung explants. A > 30% up- or down-regulation of the induction of Gli-reporter activities by Shh was chosen to select miRNAs with modulatory activities (dashed line in C). NGS, next-generation of sequencing.