Skip to main content
. 2014 Aug;51(2):273–283. doi: 10.1165/rcmb.2013-0127OC

Figure 5.

Figure 5.

miR-326 suppresses Shh-induced Gli-reporter activities by targeting Smoothened (Smo) and Gli2. (A) miR-326 suppresses the Shh-induced Gli-reporter activities at low and medium cell densities. (B) Luciferase activities of Smo–3′-UTR or Gli2–3′-UTR reporters were decreased by miR-326 but not by miR-715, an unrelated miRNA. Suppression of luciferase activity of Gli2–3′-UTR reporter depends on the miR-326 binding site being intact. (C) Levels of miR-326 in HCT116 cells transduced with miR-326 lentivirus. (D) Overexpression of miR-326 does not alter the levels of Gli2 and Smo miRNAs. (E) Gli2 and Smo protein levels are significantly reduced in miR-326 overexpression cells, as compared with cells transduced with control or miR-715 expressing lentivirus. Results are representative of three independent experiments. Error bars = SD. *P < 0.001; **P < 0.05. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.