Figure 2.

Activity of PI3-h1 and PI3-h2 promoters. A549 cells were transfected with pPI3-h1, pPI3-h2, or empty pEZX-MT01 (control) reporter plasmids. At 24 hours after transfection, cells were stimulated with IL-1β (20 ng/ml). The luciferase assay results are presented as relative activity compared with the control (empty pEZX-MT01 vector) as means (± SD) (n = 6). Fold increase was calculated after defining the activity of the empty pEZX-MT01 vector as 1. One-way ANOVA was used in comparisons. *P < 0.05. The differences between cells transfected with pPI3-h1 or pPI3-h2, as well as between the control cells and cells transfected with pPI3-h1 or pPI3-h2, were significant.