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. 2014 Aug;51(2):169–177. doi: 10.1165/rcmb.2013-0298OC

Figure 4.

Figure 4.

Altered NgBR level affects nitric oxide (NO) and reactive oxygen species (ROS) formation in PAECs. (A) NgBR overexpression in IPH PAECs increased 4-amino-5-methylamino-2′, 7′-difluorofluoresceine (DAF) fluorescence, indicating increased NO formation. (B) NgBR overexpression in IPH PAECs decreased dihydroethidium (DHE) fluorescence, indicating decreased ROS formation. (C) NgBR knockdown in control PAECs decreased DAF fluorescence and (D) increased DHE fluorescence. l-NAME, Nω-nitro-l-arginine methyl ester hydrochloride; SOD, polyethylene glycol–conjugated copper zinc superoxide dismutase. *P < 0.05 compared with empty plasmid; #P < 0.05 compared with L-NAME treated cells; †P < 0.05 compared with NS siRNA; ‡P < 0.05 compared with cells not treated with SOD. Data are shown as mean (± SE).