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. 2014 Aug;51(2):210–222. doi: 10.1165/rcmb.2013-0353OC

Figure 3.

Figure 3.

Decreased transepithelial electrical resistance (RT) and increased solute and ion permeability in MAECMs from C18 KO mice. (A) RT was significantly lower across MAECMs from C18 KO mice (0.88 ± 0.06 kΩ·cm2; n = 53) versus MAECMs from WT mice (1.86 ± 0.10 kΩ·cm2; n = 51). ***P < 0.001. (B) There was no difference in IEQ between C18 KO (6.1 ± 0.3 μA/cm2; n = 53) and WT (6.2 ± 0.2 μA/cm2; n = 51) MAECMs. (C) In vitro permeability (Papp) to 5-carboxyfluorescein of MAECMs from C18 KO mice (38 × 10−8 ± 1.3 × 10−8 cm/s; n = 3) was significantly higher versus MAECMs from WT mice (24 × 10−8 ± 0.5 × 10−8 cm/s; n = 3). **P < 0.01. (D) In vitro tetramethylrhodamine-isothiocyanate (TRITC)-dextran permeability (Papp) was significantly higher in MAECM from C18 KO mice (2.3 × 10−8 ± 0.03 × 10−8 cm/s; n = 3) versus MAECM from WT mice (1.5 × 10−8 ± 0.15 × 10−8 cm/s; n = 3). *P < 0.05. (E) PNa/PCl was not different between WT (1.37 ± 0.06; n = 7) and C18 KO (1.52 ± 0.04; n = 7) MAECM. (F) No difference in PNa/PK was found between genotypes (1.01 ± 0.01 versus 0.97 ± 0.01 in WT [n = 7] and C18 KO [n = 7] MAECMs, respectively). (G) Ion permeability was significantly higher in C18 KO compared with WT MAECMs (n = 7) for Na (***P < 0.001), Cl (*P < 0.05), and K (***P < 0.001).