Skip to main content
. Author manuscript; available in PMC: 2014 Aug 28.
Published in final edited form as: J Vasc Surg. 2008 Jan;47(1):166–172. doi: 10.1016/j.jvs.2007.09.016

Figure 3.

Figure 3

Reverse zymographic analysis of TIMP levels in the mouse thoracic aortas. Mouse thoracic aortas from wild type control, mgR/mgR, and doxycycline-treated mgR/mgR mice harvested at 6 weeks of age (n=5 mice/group). Aortic proteins were separated by electrophoresis on a 12.5% SDS-PAGE containing 1.6% gelatin, 0.16 μg/ml MMP-2. A representative reverse zymogram shows the bands representing gelatin preserved by TIMP-1 and TIMP-2 (indicated by arrows). Band intensities were measured by densitometer. Relative expression of TIMP-1 and TIMP-2 in wild type control, mgR/mgR, and doxycycline-treated mgR/mgR mouse aortas was analyzed.