FIG. 1.

Assessment of BRAF^V600E in papillary thyroid carcinoma specimens by polymerase chain reaction (PCR) and nucleotide sequencing. Hematoxylin and eosin staining of thyroid tissue is shown for (A) normal thyroid tissue and papillary thyroid carcinoma (400× original magnification). (B) Separation of PCR products of the BRAF gene by gel electrophoresis. Direct sequencing of purified PCR project shows (C) a single thymine corresponding to nucleotide 1799 representing the wild-type (WT) allele, and (D) an adenosine peak at the same sequence location demonstrating the BRAF c.1799T>A mutation that encodes the BRAF^V600E protein (right panel).