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. 2014 May 26;5(13):4746–4764. doi: 10.18632/oncotarget.2012

Figure 1. Metformin inhibited adipocyte driven growth of ID8 ovarian cancer cells.

Figure 1

3T3L1 mouse preadipocytes were differentiated into adipocytes over a period of 9 days and conditioned media (CM) was collected and used to treat ID8 cells. Mouse ovarian cancer cell line ID8 was exposed to the adipocyte CM (adipo CM) at various dilutions (0-100%) in basal media and MTT assay performed at 48 (Ai) and 72 (Aii) hours (*p < 0.05, **p < 0.01, ***p < 0.001 compared to 0, basal media). ID8 cells were exposed to 50% adipo CM in presence of 2 and 4 mM concentration of metformin. Cell proliferation was assessed by MTT assay performed at 48 (Bi) and 72 hours (Bii) (###p<0.001 compared to basal media; ***p < 0.001 metformin treated compared to adipo CM alone). ID8 cells were exposed to the 50% adipo CM or 50% CM from adipocytes that were treated with different concentrations of metformin while differentiating (adipo M2 CM: metformin 2 mM and adipo M4 CM: metformin 4 mM). Cell proliferation was assessed by MTT assay performed at 48 (Ci), and 72 (Cii) hours (###p < 0.001, #p < 0.05, ns: non-significant compared to basal media; **p < 0.01, ***p < 0.001 metformin treated compared to adipo CM alone).