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. 2014 May 27;5(13):4779–4787. doi: 10.18632/oncotarget.2029

Figure 3. Caspase-3-like protease cleavage removes a C-terminal fragment from JMJD2C.

Figure 3

(A) HeLa cells were stably transfected with full length JMJD2C (D396) tagged with FLAG at the C-terminus. The top schematic representation shows the C-terminal FLAG epitope tag of JMJD2C. Where indicated, HeLa cells were treated with 20 mM z-DEVD-fmk for 2 h before treatment and then were treated with TNF-α (25 ng/ml) plus CHX (10 μg/ml) for 6 h. Cell lysates of HeLa were analyzed by western blotting with FLAG antibody. Endogenous levels of total full-length PARP-1 and the large fragment produced by caspase-3 family protease cleavage at Asp214 were detected by PARP antibody. (B) HeLa cells were stably transfected with full length JMJD2C (D396) tagged with HA at the N-terminus. The top schematic representation shows the N-terminal HA epitope tag of JMJD2C. Treated with TNF-α (25 ng/ml) plus CHX (10 μg/ml) for 6 h, cell lysates of HeLa were analyzed by western blotting with HA antibody. The untransfected and untreated cells were set as the control. Full-length and the cleavage fragment of PARP-1 were also detected.