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. 2014 Jun 10;5(13):5113–5124. doi: 10.18632/oncotarget.2089

Figure 2. miR-137 inhibits HCC cell proliferation, migration and invasion in vitro.

Figure 2

(A) miR-137 re-expression was confirmed in SK-Hep1 and QGY-7703 cells using qRT-PCR. (B) miR-137 reduced cell viabilities in HCC. Cells were seeded onto 96-well plates and transfected with miR-137 mimics for 5 days. Cell viabilities were determined using MTT assays. (C) miR-137 suppressed colony formation in HCC. Cells stably expressing miR-137 were cultured with 400 μg/ml G418 for two weeks. The number of colonies was calculated and depicted by the histogramme. Data were represented as the mean + SEM of three independent experiments. (D, E) miR-137 inhibited cell migration and invasion in HCC. Transwell and invasion assays were performed to determine the effect of miR-137 on HCC cell migration and invasion. Representative images (left panel) and the quantification of three randomly selected fields (right panel) are shown. (F) The protein expression levels of E-cadherin, N-cadherin, vimentin, and MMP2 in SK-Hep1 and QGY-7703 cells transfected with miR-137 mimics for 24 h were examined. All *P < 0.05; ** P < 0.01.