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. 2014 Jun 11;5(13):5138–5152. doi: 10.18632/oncotarget.2094

Figure 1. Effect of ERBB3 knockdown on cell proliferation, cell cycle and apoptosis in HCT116 cells.

Figure 1

(A) Viable cells were counted 72 h after treatment with different concentration of siRNA (left) or at different time points after treatment with 5 nM siRNA (right). (B) Cell cycle distribution was analyzed with FACS 72 h after transfection with different concentration of siRNA (left) or at different time points after treatment with 5 nM of siRNA (right). Numbers in open box indicate a percent of G1 populations. (C) Western blotting was performed using equal amounts of protein extracts prepared 72 h after transfection with different concentration of siRNA (top). The apoptotic index (Parp1 cleavage) was determined by the ratio of cleaved (C) to uncleaved Parp1 (U) (bottom). (D) The time course induction of Parp1 cleavage was determined after the treatment with 5 nM of siRNA using western blotting (top) and quantified (bottom). (E) Cells were analyzed with FACS or (F) western blotting (left) and Parp1 cleavage (right) was quantified after cells were transiently transfected with Erbb3 cDNA (Erbb3) expression vector or vector only (Empty), followed by siRNA treatment for 48 h. In B, D, E and F, – denotes treatment with siCTRL and +, with siERBB3. siERBB3 group was statistically compared to siCTRL group at each point, unless otherwise indicated.