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. 2014 Jun 11;5(13):5153–5164. doi: 10.18632/oncotarget.2095

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Copyright: © 2014 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A, Western blot analysis showing the localization of HDA2 to both the nucleus (N) and cytoplasm (C) of HCT116 cells; B, In vitro immunoprecipitation analysis of the MTA1-HDAC2 interaction in the nuclear and cytoplasmic fractions of HCT116-M1 cells; C, In vitro immunoprecipitation analysis of the MTA1-HDAC2 interaction in the nuclear and cytoplasmic fractions of HCT116-M3 cells; D, In situ PLA analysis. left, the control interaction detected using mouse antibody against MTA1 and rabbit IgG; right, in situ PLA visualization of the MTA1-HDAC2 interaction in both the nucleus and cytoplasm of HCT116 cells. The white arrow shows the positive signal in the cytoplasm.