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. 2014 Jun 12;5(13):5165–5176. doi: 10.18632/oncotarget.2099

Figure 4. Ribosomal protein acetylation is induced by pan-HDACi and HDAC6-selective inhibitors.

Figure 4

(a-c) SKBR3 cells were treated with the indicated drugs for the indicated period of time. (d) SKBR3 cells were transfected with the indicated siRNAs and allowed to incubate for 72 hours. (a, c, d). The indicated western blots were performed in ribopellets, total cytoplasmic lysates, or nuclear extracts. (b) Mass spectrometry was performed on ribopellets as described in materials and methods and in Figure 6. The fold change in acetylated peptide to total peptide caused by TSA treatment is plotted. Only proteins that underwent at least a two-fold induction upon TSA treatment are shown. Error bars represent the standard error of the mean for three biological replicates.