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Comparison of the key third components of Dpo4 and pol β. τ3 (top) and the corresponding S3 (bottom) are directly related to the flexibility of the binding interface and the mobility of the interfacial water molecules. For pol β, the relaxation significantly slows down, but for Dpo4, it shows slight changes, reflecting the different binding properties of the two polymerases. However, all water at the interface for the two polymerases is still mobile.
