Figure 3.

Model reactions utilized to assess the immobilized platform. Reaction 1 is a 2-chloro-4-nitrophenyl glucose (PNP-Glc)-driven glycosyltransferase-catalyzed “reverse” reaction to produce the desired sugar nucleotide UDP-Glc. Reaction 2 is a standard glycosyltransferase-catalyzed glycosylation of the model acceptor 4Me-UMB, where the UDP-Glc from reaction 1 serves as the sugar donor in a sequential reaction. For the current study, reaction 1 occurs on the top face while reaction 2 occurs at the bottom face of the reactor membrane.