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. 2014 Jul 15;8(8):8104–8112. doi: 10.1021/nn502181k

Figure 7.

Figure 7

HPLC analysis of a representative immobilized sequential reaction containing 2.56 mM PNP-Glc and 1 mM UDP in 50 mM Tris-HCl, pH 8.5, in the upper reactant chamber injected through the membrane carrying immobilized OleD Loki on the top and bottom face into a bottom reaction chamber containing 0.56 mM 4Me-UMB in the same buffer. Flow rate for this sequential reaction was 0.5 mL/h at 25 °C for 4 h, and the bottom reaction chamber contents were subsequently analyzed by HPLC. For comparison, Figure S4 highlights a representative solution-phase sequential reaction.