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Colocalization of internalized DNA delivered by C32–122 with markers of distinct endocytic pathways in MEFs. NPC1+/+ MEFs were transfected with C32–122 polyplexes containing Cy3-labeled DNA in the presence of AF647-labeled (a) 55 ng/mL cholera toxin B or (b) 50 μg/mL transferrin. After 3 h, the cells were washed, fixed, treated with the nuclear stain Hoescht (blue), and analyzed by confocal microscopy for intracellular colocalization of DNA (green) with each marker (red).
