Fig. 5.

Cerebella of Npc1^−/− and Npc1^+/+ display comparable numbers of apoptotic GNs. (A) Cerebellar sections of PN12, PN14 and PN28 Npc1^+/+ and Npc1^−/− mice, either untreated or treated with a single injection of HPBCD at PN7, were stained with TUNEL. A representative field showing TUNEL-positive cells (red) of lobule II of PN14 Npc1^+/+ (top), Npc1^−/− (middle) and HPBCD-treated Npc1^−/− mice (bottom). Arrowheads indicate TUNEL-positive cells. Scale bar indicates 50 μm. (B) Histograms represent the number of TUNEL-positive cells (mean ± SEM; 4–5 mice/group; 3–4 sections/mouse) determined in 1 mm² regions corresponding to the crown of lobule II of Npc1^+/+ (empty bars), Npc1^−/− (full bars) and HPBCD-treated Npc1^−/− (dashed bars) mice.