Fig. 5.

Starvation-resistant flies do not suppress sleep in response to starvation. (A) Sleep was measured in flies housed on agar alone (white bar). F flies suppress sleep after fasting [F_3,234 (population×time)=33.0, P<0.001, N=119 for F and S populations], whereas no difference in sleep was observed in S flies over 14 days (R²=0.031, P>0.05). (B) Starvation resulted in reduced bout number following 24 h of fasting in F flies [ANCOVA (day×population): F_3,235=17.4, P<0.001, N=120 and 119 for F and S populations, respectively; post hoc test between the first and second day (day×population): F_1,237=18.4, P<0.001]. (C) F flies increase waking activity following 1 day of fasting upon comparison with S flies [ANCOVA (day×population): F_3,234=29.3, P<0.001, N=119 for F and S populations]. (D) F and S flies were sleep-deprived (SD) from hours 12–24 (hashed bar) during the dark period. Both F and S flies display much more robust sleep rebounds compared to undisturbed controls (P<0.001 for all groups). L/D, light (white) and dark (black).