Skip to main content
. 2014 Aug 28;10(8):e1004544. doi: 10.1371/journal.pgen.1004544

Figure 4. Insulator factors interact upon DNA binding.

Figure 4

(A) Native agarose band-shift assay of BEAF32-DNAtudor, and higher order complexes. Lane 1, 476 bp DNAtudor; lane 2, DNAtudor incubated with BEAF32; lane 3, DNAtudor incubated with CP190; lane 4, DNAtudor incubated with BEAF32 and CP190; lane 5, DNAtudor incubated with Chromator; lane 6, DNAtudor incubated with BEAF32 and Chromator; lane 7, DNAtudor incubated with CP190-C; lane 8, DNAtudor incubated with BEAF32 and CP190-C; lane 9, DNAtudor incubated with Chromator-C; lane 10, DNAtudor incubated with BEAF32 and Chromator-C; lane 11, DNAtudor incubated with CP190 and Chromator; lane 12, DNAtudor incubated with CP190-C and Chromator-C; lane 13, DNAtudor incubated with BEAF32, CP190 and Chromator. Band 1 represents DNAtudor. Band 2 represents the complex between BEAF32 and DNAtudor. Band 3 represents the BEAF32/DNAtudor complex super-shifted by binding of CP190, Chromator, Chromator-C, or the addition of both CP190 and Chromator. The shift of band 1 requires the presence of BEAF32. Protein concentrations used: BEAF32 (400 nM), CP190 (50 nM), CP190-C (50 nM), Chromator (100 nM), Chromator-C (100 nM). (B) Native agarose band-shift assay of MBP-DNAtudor. This experiment used the same protein mixes and concentrations as those used in (A) but replacing BEAF32 by MBP. No shifted band is apparent.