Figure 15. Volume differences between the interface fields of a wildtype rap1a/raf complex and a rap1a/raf complex with electrostatic nullifications in raf residues.

The red arrows indicate amino acids in rap1a that are associated with decreased electrostatic complementarity with barstar when they are nullified. The green arrow indicates an amino acid below the prediction threshold that is known to influence specificity. The white arrow indicates an open prediction.