Figure 3. Fbh1 inhibits the strand-exchange reaction at early steps, but stimulates it at the late steps.

(A) Schematic of the three-strand exchange reactions. Fbh1 (90 nM) was added at the indicated step, (i)–(viii). (B) Gel image of three-strand exchange reactions. (C) Quantitation of results in panel B. Each mean value and SE was obtained from three independent experiments. (D) Three-strand exchange reactions were carried out with the indicated incubation times after addition of Swi5-Sfr1 (5–30 min). (E) Quantitation of results in panel D. Each mean value and SE was obtained from three independent experiments.