Skip to main content
. 2014 Aug 28;10(8):e1004542. doi: 10.1371/journal.pgen.1004542

Table 1. Summary of the outcomes of single-DSB induction (%).

Strain NHEJ/SCC HRR Minichromosome loss
wild-type 10.3±2.0 72.9±7.3 13.8±4.5
rad51Δ a 16.7±0.6 19.8±1.8 63.5±2.0
swi5Δ a 33.1±2.6 36.4±2.3 30.5±2.0
sfr1Δ a 25.5±3.4 43.0±2.4 31.5±2.6
rad57Δ a 19.8±3.3 37.4±4.4 42.8±4.5
rqh1Δ 70.4±1.7 12.9±0.3 16.7±0.3
srs2Δ 42.7±2.3 43.0±2.1 12.8±1.0
fbh1Δ 43.4±3.3 52.5±0.7 2.4±0.7
rad51Δ fbh1Δ 17.5±2.0 5.2±0.0 77.3±0.0
swi5Δ fbh1Δ 32.2±7.6 62.7±1.0 3.9±0.1
sfr1Δ fbh1Δ 33.1±2.7 57.1±4.0 4.6±0.9
rad57Δ fbh1Δ 56.9±1.6 36.3±0.1 5.5±0.1

Colonies that arose after HO induction were classified into three categories: NHEJ, HRR, and minichromosome loss, based on phenotypic and PFGE analyses. The average frequencies (total segregants = 100%) and standard errors were determined from the data sets in Tables S1, S2, S3.

a

Data are from Akamatsu et al. (2007) [8].

NHEJ, non-homologous end-joining; SCC, sister chromatid conversion; HRR, homologous recombination repair; PFGE, pulsed-field gel electrophoresis.