Table 1. Summary of the outcomes of single-DSB induction (%).
Strain | NHEJ/SCC | HRR | Minichromosome loss |
wild-type | 10.3±2.0 | 72.9±7.3 | 13.8±4.5 |
rad51Δ a | 16.7±0.6 | 19.8±1.8 | 63.5±2.0 |
swi5Δ a | 33.1±2.6 | 36.4±2.3 | 30.5±2.0 |
sfr1Δ a | 25.5±3.4 | 43.0±2.4 | 31.5±2.6 |
rad57Δ a | 19.8±3.3 | 37.4±4.4 | 42.8±4.5 |
rqh1Δ | 70.4±1.7 | 12.9±0.3 | 16.7±0.3 |
srs2Δ | 42.7±2.3 | 43.0±2.1 | 12.8±1.0 |
fbh1Δ | 43.4±3.3 | 52.5±0.7 | 2.4±0.7 |
rad51Δ fbh1Δ | 17.5±2.0 | 5.2±0.0 | 77.3±0.0 |
swi5Δ fbh1Δ | 32.2±7.6 | 62.7±1.0 | 3.9±0.1 |
sfr1Δ fbh1Δ | 33.1±2.7 | 57.1±4.0 | 4.6±0.9 |
rad57Δ fbh1Δ | 56.9±1.6 | 36.3±0.1 | 5.5±0.1 |
Colonies that arose after HO induction were classified into three categories: NHEJ, HRR, and minichromosome loss, based on phenotypic and PFGE analyses. The average frequencies (total segregants = 100%) and standard errors were determined from the data sets in Tables S1, S2, S3.
Data are from Akamatsu et al. (2007) [8].
NHEJ, non-homologous end-joining; SCC, sister chromatid conversion; HRR, homologous recombination repair; PFGE, pulsed-field gel electrophoresis.