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Susceptibility of the C-terminal V5 tag to proteolysis as a measure of protein orientation. Western blotting shows that Proteinase K immediately digests the C-terminal V5 epitope of PcaK in detergent micelles (Protein) and after reconstitution into proteoliposomes (PL), suggesting that the C-terminal of the reconstituted protein is exposed at the proteoliposome exterior. Time in minutes.
