Table 2. Details of gene targets.
| Gene | Gene IDa | Chromosome Number | Primer Sequence (5′-3′) | Amplicon size (bp)b | Sequence start 5′c | Fragment Length (bp)d |
| GPI * † | Tc00.1047053506529.508 | 6 | CGCCATGTTGTGAATATTGG (20) | 405 | 21 | 365 |
| GGCGGACCACAATGAGTATC (20) | ||||||
| HMCOAR * † | TC00.1047053506831.40 | 32 | AGGAGGCTTTTGAGTCCACA (20) | 554 | 21 | 514 |
| TCCAACAACACCAACCTCAA (20) | ||||||
| RHO1 * † | Tc00.1047053506649.40 | 8 | AGTTGCTGCTTCCCATCAAT (20) | 455 | 21 | 415 |
| CTGCACAGTGTATGCCTGCT (20) | ||||||
| Tc MPX * † | Tc00.1047053509499.14 | 22 | ATGTTTCGTCGTATGGCC (18) | 678 | 109 | 505 |
| TGCGTTTTTCTCAAAATATTC (21) | ||||||
| LAP * | Tc00.1047053508799.240 | 27 | TGTACATGTTGCTTGGCTGAG (21) | 444 | 22 | 402 |
| GCTGAGGTGATTAGCGACAAA (21) | ||||||
| SODB * | Tc00.1047053507039.10 | 35 | GCCCCATCTTCAACCTT (17) | 313 | 18 | 266 |
| TAGTACGCATGCTCCCATA (19) | ||||||
| RB19 * | Tc00.1047053507515.60 | 29 | GCCTACACCGAGGAGTACCA (20) | 408 | 49 | 340 |
| TTCTCCAATCCCCAGACTTG (20) | ||||||
| GPX | Tc00.1047053511543.60 | 35 | CGTGGCACTCTCCAATTACA (20) | 360 | 21 | 321 |
| AATTTAACCAGCGGGATGC (19) | ||||||
| PDH | Tc00.1047053507831.70 | 40 | GGGGCAAGTGTTTGAAGCTA (20) | 491 | 21 | 451 |
| AGAGCTCGCTTCGAGGTGTA (20) | ||||||
| GTP | Tc00.1047053503689.10 | 12 | TGTGACGGGACATTTTACGA (20) | 561 | 21 | 521 |
| CCCCTCGATCTCACGATTTA (20) | ||||||
| SODA | Tc00.1047053509775.40 | 21 | CCACAAGGCGTATGTGGAC (19) | 300 | 20 | 263 |
| ACGCACAGCCACGTCCAA (18) | ||||||
| STPP2 | Tc00.1047053507673.10 | 34 | CCGTGAAGCTTTTCAAGGAG (20) | 409 | 21 | 369 |
| GCCCCACTGTTCGTAAACTC (20) | ||||||
| Met-II | TC00.1047053510889.280 | 6 | TCATCTGCACCGATGAGTTC (20) | 700 | 51 | 389 |
| CTCCATAGCGTTGACGAACA (20) |
*Gene fragments included in the 7 loci MLST scheme;
†
Gene fragments included in the reduced 4 loci MLST scheme;
a
Gene ID: GenBank access number for the complete gene in the CL-Brener strain;
b
Amplicon size refers to the sequence size of the gene fragment including the primers regions;
c
5′ starting position: indicates the position where the analyzed sequence starts, counting from the first base of the amplicon;
d
Fragment Length refers to the sequence length used for the analyses (the analyzed fragments do not include the primer regions).