Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 1;25(17):2542–2555. doi: 10.1091/mbc.E14-01-0014

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Rorbach et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 7: — Mitochondrial ribosome integrity upon siRNA depletion of MRM2 or MRM3. (A) The mitochondrial ribosome profile in MRM2-depleted cells. Total cell lysates of control HeLa cells transfected with an unrelated siRNA or HeLa cells transfected with siRNA to MRM2 were separated on a 10–30% (wt/vol) isokinetic sucrose gradient. Fractions obtained for control and RNAi-treated cells were analyzed by Western blotting using antibodies to mt-LSU (MRPL3, MRPL12) and mt-SSU (MRPS18B). (B) Quantification of the gradient distribution of mitoribosomal components upon MRM2 silencing. Western blot signals for MRPL3, MRPL12, and MRPS18B for control and MRM2-depleted cells were quantified using ImageQuant software. The y-axis represents the fraction of the total signal relative to control cells. n = 3. All significant value changes are indicated; *p < 0.05, **p < 0.01, ***p < 0.001; two-tailed unpaired Student's t test. Error bars, 1 SD. (C) The mitochondrial ribosome profile in MRM3-depleted cells. Total cell lysates of control HeLa cells or HeLa cells transfected with siRNA to MRM3 were analyzed as per A. (D) Quantification of the gradient distribution of mitoribosomal components upon MRM2 silencing. Western blot signal was quantified and analyzed as per B.