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. 2014 Sep 1;25(17):2634–2643. doi: 10.1091/mbc.E13-12-0716

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© 2014 Hagstrom, Ma, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — The docking complex and receptor recycling machinery are necessary for proper localization of Pex7. (A) Differential centrifugation of Δpex14, Δpex4, and Δpex2 cells grown in methanol for 4 h. The postnuclear supernatant (PNS) was centrifuged at 200,000 × g for 1 h. Equivalent volumes from the resulting supernatant (S200) and pellet (P200) fractions were analyzed by Western blot. (B) Protease protection assays of P200 pellet fractions from A.