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. 2014 Sep 1;25(17):2634–2643. doi: 10.1091/mbc.E13-12-0716

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© 2014 Hagstrom, Ma, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Pex2 and Pex4 are not essential for Pex7 monoubiquitination. (A, B) Strains overexpressing His-myc-Ub (K48R) with the indicated pex deletions (A) or point mutations (B) were induced overnight in methanol. Equal-OD samples were collected and analyzed by immunoblotting for the presence or absence of Pex7 ubiquitination. (C) Pex7 levels were compared in Δpex4 + pex20 (K19R) cells after overnight growth in methanol. (D) Differential centrifugation of Δpex4 + pex20 (K19R) cells grown in methanol for 4 h. (E) Protease protection assay of P200 in (D). In D and E, long and short exposures are shown for α-HA. Asterisks indicate ubiquitinated HA-Pex7.