FIGURE 7:

Coexpression of HIF-1α, Twist, and Bmi1 in renal biopsies from patients with CKD. (A) Bmi1 immunostaining in renal biopsy tissues from patients with IgAN (original magnification, 200×). (a) Negative control. (b) Tissue from IgAN kidney, with 1+ staining (≤25% cells positive/visual field). (c) Representative photographs from IgAN kidneys with 2+ staining (25–50% cells stained positive/visual field). (d) Representative photographs from IgAN kidneys with 3+ staining (>50% cells stained positive/visual field). Arrows highlight cells with nuclear Bmi1 staining. Top left insets, higher-magnification images. (B) Scatter plot with fitted values intervals for tubular expression of Bmi1 and tubulointerstitial fibrosis. (C) Representative pictures of the immunohistochemistry analyses of HIF-1α, Twist, and Bmi1 in consecutive sections of renal tissues from patients with IgAN (original magnification, 200×). Top left insets, higher-magnification images. (D) Scatter plot with fitted values intervals (percentage of positive cells) for tubular expression of Bmi1 and HIF-1α (left) and Bmi1 and Twist (right).