Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 1;25(17):2660–2668. doi: 10.1091/mbc.E14-04-0896

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Deng et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 1: — Slf1 physically interacts with Skb1. (A) Silver-stained SDS-polyacrylamide gel of proteins isolated from skb1-3HA and untagged control strains. LC-MS/MS identified Slf1 specifically in the skb1-3HA sample. (B) Coimmunoprecipitation of endogenously tagged Skb1-3HA and Slf1-mEGFP from fission yeast cells using anti-HA beads. (C) Coimmunoprecipitation of 6His-Slf1 and Skb1-13myc. 6His-Slf1 was induced for 20 h using the pREP3X-6His-slf1 plasmid and isolated using nickel-agarose chromatography. (D) Skb1 and Slf1 physically interact in the yeast two-hybrid assay. Transformants were selected on double dropout (DDO) plates, and interactions were tested by growth on quadruple dropout plus aureobasidin A (QDO/A) plates.