FIGURE 6:

Slf1 is a novel mitotic inducer. (A) Length of dividing, septated cells of the indicated strains (mean ± SD; n > 50 for each value). (B) Phenotype of Slf1 overexpression. Images of Blankophor-stained cells are inverted single focal planes from deconvolved Z-series. The indicated plasmids were transformed and induced for 36 h at 25°C. Note the abnormal cell morphology in 6His-Slf1–overexpressed cells. Scale bar, 5 μm. (C) Slf1 overexpression is lethal. Cells transformed with pREP3X or pREP3X-6His-slf1 were spotted onto EMM-Leu plate or EMM-Leu plate supplemented with 10 μg/ml thiamine with 10× serial dilutions. Plates were incubated at 32°C for 4 d.