FIGURE 4:

DNA looping occurs at the FLO11 locus. (A) Binding of three activators at FLO11 for three activators (Flo8, Mfg1, and Mss11) shows equal binding in the promoter and terminator. The binding of two repressors (Sok2 and Sfl1) shows higher binding in the promoter. (B) Relative binding of all of the tagged TFs that bind in the promoter of FLO11 correlates with the function of the TF. The y-axis is the phenotypic score for haploid invasive growth for knockouts of each protein, with negative scores meaning that the mutant has a deleterious effect and positive scores meaning that the mutant has an enhanced effect. The x-axis represents the relative binding of the TF in the promoter relative to the terminator. (C) Looping between promoter and terminator will occur when activators are bound and FLO11 is expressed, whereas when repressors are bound, there is no looping and no FLO11 expression. (D) Looping between the FLO11 promoter and terminator depends on nitrogen starvation and is abolished in a Flo8 mutant strain. Error bars are shown as SD among three biological replicates.