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. 2014 Aug 28;9(8):e105344. doi: 10.1371/journal.pone.0105344

Figure 10. TPP co-localizes with mitochondria after 90 min.

Figure 10

MCF7, MDA-MB-231, and T47D cells were incubated with CF-labeled TPP (green) for 90 min, stained with the mitochondrial detection dye Mito-ID (purple) and then imaged using confocal microscopy. A proportion of internalized TPP co-localizes with mitochondria in all three tumor cell lines (Pearson’s coefficient: MCF7 r = 0.855, MDA-MB-231 r = 0.585, T47D r = 0.813). The Manders’ M1 coefficient was used to estimate the proportion of total TPP that co-localizes with mitochondria (40% in MCF7 cells, M1 = 0.407; 44% in MDA-MB-231 cells, M1 = 0.443). In contrast, 14% of TPP was co-localized to mitochondria in T47D cells (M1 = 0.141). These findings correlate with the differential Hsp70 membrane expression levels of the respective cancer cell lines. Images are representative single frames. Objective 63×; scale bar 10 µm.