Figure 9. MAPK inhibitors alter CK/CHK production without reducing viral protein production in either cell type.

As MAPK inhibition did not substantially block FLUA protein production the decreased CK/CHK production is not due to a direct anti-viral effect of the inhibitors. Representative Western blots of FLUA proteins. Densitometry values shown are the percent protein produced by the inhibited cells compared to the infected, uninhibited cells at the corresponding time points; mean percentage ± SEM, n = 3 independent experiments. FO3–FLUA only 3 h pi; JEI3–both JNK and ERK inhibition 3 h pi; JI3–JNK inhibition 3 h pi; EI3–ERK inhibition 3 h pi; NoF–mock infected cells with no inhibitors (treated with DMSO); FO6–FLUA only 6 h pi; JEI6–both JNK and ERK inhibition 6 h pi; JI6–JNK inhibition 6 h pi; EI6–ERK inhibition 6 h pi.