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. 2014 Aug 28;9(8):e105901. doi: 10.1371/journal.pone.0105901

Figure 2. Biofilm structure of S. fredii SMH12 and derivatives on glass surfaces: reconstruction of the Z-stacks and measure of the surface coverage.

Figure 2

Main fluorescence value of the wild-type strain was arbitrarily given a value of 100. Averages and standard deviations of five randomized optical fields per strain corresponding to two independent experiments are shown. The asterisks indicate a significant different at the level α = 5% with respect to wild-type strain by using the Mann-Whitney non-parametrical test. Left side corresponds to cultures without flavonoids. Right side corresponds to cultures with inducing flavonoid. A. SMH12. B. SVQ648. C. SMH12 (pME6863). D. SMH12 (pME6000). Bar, 20 µm. SMH12: wild-type, SVQ648: nodD1 mutant, SMH12 (pME6863): lactonase strain. SMH12 (pME6000): carrying the empty plasmid.