. 2014 Aug 28;9(8):e105901. doi: 10.1371/journal.pone.0105901

Table 1. Resistance phenotypes: Gm^R, Km^R, Nx^R, Ap^R and Tc^R, gentamicin, kanamycin, nalidixic acid, ampicillin and tetracycline, respectively.

Strain or plasmid	Relevant properties	Reference
Agrobacterium tumefaciensNT1 (pZRL4)	A.tumefaciens without pTiC58;with pZRL4, which carries thefusion traG::lacZ and the gene traR, Gm^R	[13]
Escherichia coliDH5α	SupE44, ΔlacU169, 5hsdR17,recA1, endA1, gyrA96, thi-1, relA1, Nx^R	[25]
Sinorhizobium fredii SMH12	Wild-type strain, Ap^R	[40]
SMH12 (pME6000)	SMH12 with the plasmid pME6000, Tc^R	This work
SMH12 (pME6863)	SMH12 with the plasmid pME6863, whichcarries the lactonase gene, Tc^R	This work
SMH12 (pMP2463)	SMH12 with the plasmid pMP2463, whichcarries the gene of the GFP, Gm^R	This work
SMH12 (pMP6000)(pMP2463)	SMH12 with the plasmids pME6000 and pMP2463,which carry the empty plasmid and thegreen fluorescent protein, respectively, Tc^R Gm^R	This work
SMH12 (pMP6863)(pMP2463)	SMH12 with the plasmids pME6863 and pMP2463,which carry the gene that encode the lactonaseand the green fluorescent protein, respectively, Tc^R Gm^R	This work
SVQ648	SMH12 nodD1::lacZ-Gm^R	This work
SVQ648 (pMP2463)	SVQ648 with the plasmid pMP2463, whichcarries the gene of the GFP, Gm^R	This work
pBBR1MCS-5	Broad-host-range cloning vector, Gm^R	[41]
pK18mobsacB	Cloning vector (suicide in rhizobia), Km^R	[42]
pME6000	Broad-host-range cloning vector, Tc^R	[43]
pME6863	pME6000::aiiA, plasmid carrying thelactonase gene, Tc^R	[44]
pMP2463	pBBR-MCS-5::egfp-1, plasmid carryingthe green fluorescent protein gene, Gm^R	[45]
pMUS534	Plasmid pK18mob derivative containingnodD1::lacZ-Gm^R	[46]
pRK2013	Helper plasmid, Km^R	[47]

Table 1. Resistance phenotypes: GmR, KmR, NxR, ApR and TcR, gentamicin, kanamycin, nalidixic acid, ampicillin and tetracycline, respectively.