Table 1. Strains, plasmids and putative genes studied in the work.
Name | Description | Source/reference |
Strains | ||
P. aeruginosa SJTD-1 | Host strain isolated from oil-contaminated soil, Wild Type | This study |
MutalkB1/MutalkB2/MutalkB1&2 | AlkB-deleted mutants of P. aeruginosa SJTD-1 | This study |
MutP450-1/MutP450-2/MutP450-1&2 | P450-deleted mutants of P. aeruginosa SJTD-1 | This study |
MutalmA | AlmA-deleted mutants of P. aeruginosa SJTD-1 | This study |
Plasmids | ||
pRKaraRed | Broad-host-range, λ-Red proteins expression vector, TetR | [25] |
Genes * | ||
4712 | alkane-1 monooxygenase, AlkB1 | This study |
3623 | alkane-1 monooxygenase, AlkB2 | This study |
5482 | putative cytochrome P450 hydroxylase, P450–1 | This study |
4609 | putative cytochrome P450 hydroxylase, P450–2 | This study |
3206 | monooxygenase, flavin-binding family, AlmA-like protein | This study |
*Gene numbers presented in RAST server.