Table 3. RT-LAMP-LFD Detection of FMDV in air samples collected with 2 different portable air samplers (Biobadge 100 and Biocapture 650).
| Sample collection/days post infection | |||||||
| Species infected | Air sampling instrument used | Assay utilised | 0 | 1 | 2 | 3 | 4 |
| Pig | Biobadge (short Run ∼3 hrs) | RT-qPCR (Ct Value) | No Ct | 25.8 | 23.52 | 30.07 | 27.66 |
| LAMP-LFD | − | + | + | + | + | ||
| Biobadge (long run ∼20 hrs) | RT-qPCR (Ct Value) | No Ct | 20.74 | 23.51 | 24.02 | 28.19 | |
| LAMP-LFD | − | + | + | + | + | ||
| Biocapture (∼30 mins) | RT-qPCR (Ct Value) | No Ct | 22.41 | 23.49 | 23.68 | 29.74 | |
| LAMP-LFD | − | + | + | + | + | ||
| Cattle | Biocapture (∼30 mins) | RT-qPCR (Ct Value) | No Ct | No Ct | 33.84 | No Ct | No Ct |
| LAMP-LFD | − | − | + | − | − | ||
| Sheep | Biocapture (∼30 mins) | RT-qPCR (Ct Value) | No Ct | 35.78 | 37.31 | No Ct | No Ct |
| LAMP-LFD | − | + | − | − | − | ||
RNA was extracted and analysed using the RT-qPCR assay, with Ct values being displayed.
Raw air samples were applied to the RT-LAMP-LFD assay, and analysed using the LFD. Air samples were collected from day 0 (day of inoculation), every day, until day 4. Species sampled were pigs, cattle and sheep.