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. 2014 Aug 28;9(8):e106040. doi: 10.1371/journal.pone.0106040

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© 2014 Wani et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Fifty ng phosphorylated GST-CTD was incubated with 3.4 µg GST (lane 1), 0.2 µg SCP1 (lane 2), 0.2 µg, 1 µg, or 5 µg wild-type human Ssu72 (lanes 3–5), or 5 µg mutant (C12S) Ssu72 (lane 6), followed by Immunoblotting using the phospho CTD–specific antibodies 3E10 (Ser2P), 3E8 (Ser5P), and 4E12 (Ser7P) and an antibody against the CTD (8WG16). (B) Purified human Pol II was incubated with wild-type (lane 1) or mutant (C12S) Ssu72 (lane 2) and analyzed by Western blotting using phospho CTD–specific antibodies 3E10 (Ser2P), 3E8 (Ser5P), 4E12 (Ser7P), and 6D7 (Thr4P).