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. 2014 Aug 28;9(8):e106001. doi: 10.1371/journal.pone.0106001

Figure 3. AS validation of 14 selected genes by RT-PCR.

Figure 3

The splicing patterns of selected AS genes were analyzed using RT-PCR to validate the RNA sequencing results. Electrophoretic analysis of amplified products from RT-PCR with 3% agarose gel. M, DL500 marker, and the size of specific bands are indicated. The numbers 1–14 represent the PCR products of contig name comp 65400_c0, comp 62996_c0, comp69107_c0, comp65089_c0, comp67573_c0, comp67051_c1, comp68099_c0, comp68043_c2, comp57809_c0, comp66555_c0, comp66894_c0, comp63097_c0, comp64465_c1, and comp5406_c0, respectively.