Figure 2. Representative Cluster Analysis of the en face db/db vascular wall sample.

The Raman distribution image of the db/db sample obtained by integration of the band in the 2800–3100 cm⁻¹ range (A), the CA results (K-means, Manhattan distance, 5 classes; B) and the complementary average spectra of classes (spectra were normalized to the 1011 cm⁻¹ band and offset in order to emphasize the differences between them, the Raman intensity in the 2700–3200 cm⁻¹ range is 3-fold magnified relatively to the fingerprint region, C).