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. 2014 Jul 18;10(4):1786–1792. doi: 10.3892/mmr.2014.2394

Figure 1.

Figure 1

Effect of hyperoxia on C5aR expression levels in the mouse lung. To investigate the effect of hyperoxia on the expression levels of C5aR, Balb/c mice were ventilated with 100% oxygen for 36 h. (A) Following treatment with 100% oxygen, the C5aR mRNA expression levels in the mouse tissue were assessed by reverse transcription polymerase chain reaction. Hyperoxia significantly increased the expression levels of C5aR in the lung tissue. (B) C5aR expression levels in BALF macrophages from non-treated or 100% oxygen-treated mice were also assessed by flow cytometry. The C5aR expression levels in BALF macrophages from 100% oxygen-treated mice were significantly increased. Data are expressed as the mean ± SD (n=5). **P<0.01, hyperoxia vs. control. C5aR, C5a receptor; BALF, bronchoalveolar lavage fluid; FITC, fluorescein isothiocyanate.