Figure 6.
C5aRA attenuated hyperoxia-induced MPO activity, and CD68 and F4/80 expression in the lung tissue. Balb/c mice were ventilated with 100% oxygen for 36 h with or without C5aRA treatment, (A) MPO activity was measured by enzyme-linked immunosorbent assay, and the relative levels of (B) CD68 and (C) F4/80 mRNA expression were detected by reverse transcription polymerase chain reaction. Hyperoxia induced MPO activity, and CD68 and F4/80 mRNA expression in the lung tissue; the hyperoxia-induced MPO activity, and CD68 and F4/80 mRNA expression were significantly suppressed by the treatment with C5aRA. Data are expressed as mean ± SD (n=5). **P<0.01, hyperoxia vs. control; ##P<0.01, hyperoxia + C5aRA vs. hyperoxia. C5aRA, C5a receptor antagonist; MPO, myeloperoxidase; mRNA, messenger ribonucleic acid.