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Phloroglucinol induces the apoptosis of HT-29 colon cancer cells. The percentages of apoptotic and necrotic cells were determined using an Annexin V and Dead Cell assay. Cells in the early stages of apoptosis showed Annexin V-positive and 7-AAD-negative staining, and cells in late apoptosis showed Annexin V-positive and 7-AAD-positive staining.
